Application of the ExperionTM Automated Electrophoresis System to Glycoprotein Visualization and Analysis

Identical amounts of each protein were analyzed by SDSPAGE and with the Experion system (Bio-Rad Laboratories). For SDS-PAGE, samples were prepared by mixing equal volumes of protein sample and 2x Laemmli sample buffer with or without 5% β-mercaptoethanol. Following separation on CriterionTM Tris-HCl 4–20% precast gels, gels were stained with Bio-SafeTM colloidal Coomassie Blue stain and imaged using a GS-800TM densitometer and Quantity One® software (Bio-Rad Laboratories). For Experion analyses, samples were combined with reducing or nonreducing sample buffer and separated using the Experion Pro260 analysis kit according to the protocol described in the instruction manual.